Use of Polyphosphates and Soluble Pyrophosphatase Activity in the Seaweed Ulva pseudorotundata

  1. Vergara, Juan J.
  2. Herrera-Pérez, Patricia
  3. Brun, Fernando G.
  4. Pérez-Lloréns, José Lucas
  1. 1 Universidad de Cádiz
    info

    Universidad de Cádiz

    Cádiz, España

    ROR https://ror.org/04mxxkb11

Revista:
Oceans

ISSN: 2673-1924

Año de publicación: 2020

Volumen: 1

Número: 4

Páginas: 343-354

Tipo: Artículo

DOI: 10.3390/OCEANS1040023 GOOGLE SCHOLAR lock_openAcceso abierto editor

Otras publicaciones en: Oceans

Resumen

The hydrolytic activity of different types of polyphosphates, and the induction of solublepyrophosphatase (sPPase; EC 3.6.1.1) activity have been assessed in cell extracts of nutrientlimited green seaweed Ulva pseudorotundata Cormaci, Furnari & Alongi subjected to differentphosphorus regimes. Following a long period of nutrient limitation, the addition of different typesof (poly)phosphates to artificial seawater enhanced growth rates on fresh weight and area, but noton dry weight bases. Chlorophyll and internal P content were affected by P supply. In contrast,internal soluble reactive P was kept low and was little affected by P additions. Soluble protein contentincreased in all treatments, as ammonium was added to prevent N limitation. The C:N:P atomicratio revealed great changes depending on the nutrient regime along the experiment. Cell extractsof U. pseudorotundata were capable of hydrolyzing polyphosphates of different chain lengths (pyro,tripoly, trimeta, and polyphosphates) at high rates. The sPPase activity was kept very low in P limitedplants. Following N and different kind of P additions, sPPase activity was kept low in the control,but slightly stimulated after 3 days when expressed on a protein basis. The highest activities werefound at the end of the experiment under pyro and polyphosphate additions (7 days). The importanceof alternative P sources to phosphate and the potential role of internal soluble pyrophosphatases inmacroalgae are discussed.

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