Assessing PAH detoxification in the clam Ruditapes philippinarum
- Carmen Morales-Caselles 1
- M. Laura Martín-Díaz 1
- Rocío Antón-Martín 2
- Inmaculada Riba 1
- Carmen Sarasquete 1
- T. Ángel Del Valls 2
- 1 Instituto de Ciencias Marinas de Andalucía (ICMAN-CSIC), Avda. República Saharaui s/n, Puerto Real 11510 Cádiz, Spain
- 2 UNESCO UNITWIN/UNICOP, Facultad de Ciencias del Mar y Ambientales, Universidad de Cádiz, Polígono Río San Pedro s/n, Puerto Real 11510 Cádiz, Spain
Year of publication: 2008
Type: Conference paper
Abstract
The PAH detoxification system in the clam Ruditapes philippinarum was studied by analyzing the kinetics of phase I and phase IIdetoxification enzymes in digestive gland. Levels of phase I cytochrome P450A (CYP1A)-like enzymes were measured as ethoxyresorufin O-deethylase (EROD) activity and phase II bygluthatione-S-transferase. Analyses were performed on clamsexposed to PAH-contaminated sediments after 7, 14, 21 and 28days of exposure. Contaminated sediments were collectedfrom two areas of the Spanish coast affected acutely (GalicianCoast) and chronically (Bay of Algeciras) by oil spills; bioassayswere performed under both laboratory and field conditions. Sediments from the selected sites were chemically characterizedand the data obtained were compared with the biomarkerresults. A kinetic approach to the induction of these biomarkers potentially related to PAH detoxification in clam wasperformed.