Características histoquímicas de los ovocitos vitelogénicos del atún rojo, Thunnus thynnus L.

  1. Sarasquete, Carmen 2
  2. González de Canales, M. Luisa 3
  3. Piñuela, Carmen 2
  4. Muñoz-Cueto, J. Antonio 3
  5. Rendón, Carmen 3
  6. Mañanós, Evaristo L. 1
  7. Rodríguez-Gómez, F.J. 3
  8. Pascual, Emilio 2
  1. 1 Instituto de Acuicultura de Torre de la Sal
    info

    Instituto de Acuicultura de Torre de la Sal

    Castelló de la Plana, España

    ROR https://ror.org/00xk8t981

  2. 2 Instituto de Ciencias Marinas de Andalucía
    info

    Instituto de Ciencias Marinas de Andalucía

    Cádiz, España

    ROR https://ror.org/04qayn356

  3. 3 Universidad de Cádiz
    info

    Universidad de Cádiz

    Cádiz, España

    ROR https://ror.org/04mxxkb11

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Journal:
Ciencias marinas

ISSN: 0185-3880,  2395-9053

Year of publication: 2002

Volume: 28

Issue: 4

Pages: 419-431

Type: Article

DOI: 10.7773/CM.V28I4.230 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Ciencias marinas

Abstract

Very little information is available with respect to dynamic aspects of oocyte development and to yolk composition of tuna species. Since histological approaches can offer considerable information about the ovarian development of fish, and because different cytochemical/immunohistochemical tests are suitable tools for proving the presence of different macromolecules inseveral organs/tissues/cells, they can be good biomarkers of the reproductive mechanism in fish. The present study investigates the presence and distribution of carbohydrates, proteins, glycoconjugates, lipids, calcium, iron and vitellogenin in the bluefintuna, Thunnus thynnus oocytes during the synthesis of yolk (globules and granules) in the vitellogenic phase. During the initialvitellogenic phase, oocytes (diameter: 249 ± 21.87 mm) contain oil globules (or vacuoles) which consist exclusively of neutrallipids. During this phase very small yolk granules appear around the cytoplasm. Glycolipids, phospholipids, glycoproteins (N-acetyl-D-galactosamine –GalNAc– sugar residues), calcium, proteins rich in tyrosine, tryptophan, lysine, arginine, cysteine andcystine, as well as vitellogenin are the main constituents of the yolk granules and the follicular envelope of these real vitellogenicoocytes (diameter: 386 ± 79.04 mm). The intergranular cytoplasm of the vitellogenic oocytes also contain glycogen andglycoconjugates with Mannose –Man– and/or Glucose –Glc–; N-acetyl-D-galactosamine –GalNAc–; L-Fucose –Fuc– and N-acetyl-D-glucosamine –GlcNAc– and/or sialic acid –NANA– sugar residues. The zona radiata is mainly composed of proteins; in this cellular layer, scarce neutral glycoproteins and lipids (phospholipids/glycolipids) are also present.

Data source: Dialnet