Phosphatase activity in salt-ponds of the Bay of Cadiz

  1. M.D. Frutos 1
  2. A. Gómez Parra 1
  3. J. Blasco 2
  1. 1 Universidad de Cádiz
    info

    Universidad de Cádiz

    Cádiz, España

    ROR https://ror.org/04mxxkb11

  2. 2 Instituto de Ciencias Marinas de Andalucía
    info

    Instituto de Ciencias Marinas de Andalucía

    Cádiz, España

    ROR https://ror.org/04qayn356

Journal:
Ciencias marinas

ISSN: 0185-3880 2395-9053

Year of publication: 2004

Volume: 30

Issue: 3

Pages: 403-416

Type: Article

DOI: 10.7773/CM.V30I3.332 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Ciencias marinas

Abstract

Acid and alkaline phosphatase activities (E.C.3.1.3.2 and E.C.3.1.3.1, respectively) were measured in seawater and sediments from different sites of a salt-pond of the Bay of Cádiz. The profiles of enzymatic activity relative to depth were also analysed. Initially, the optimum conditions (pH, temperature, substrate concentration and amount of sample) for determining enzymatic activities were studied. The apparent activation energies for alkaline phosphatase activity in seawater and sediments were 53.8 and 48.6 kJ mol¯¹, respectively. The Km and Vmax were 10.6 mM and 89 μg of 4-nitrophenol g¯¹ h¯¹ and 8.42 mM and 145 μg of 4-nitrophenol L¯¹ h¯¹ for sediments and seawater, respectively. Both types of enzymatic activity reached high values in seawater and sediments; in the confined areas of the salt-pond the values were around 100 μg of 4-nitrophenol L¯¹ h¯¹ for seawater and higher for sediments. In general, clear seasonal evolutions for both types of enzymatic activity were found in the seawater, with maxima at the end of summer and minima in winter. No seasonal pattern was evident in the sediment. Stratification of phosphorus regeneration was observed in the sediment column but there was no stratification at depths greater than 15 cm.