Ejercicio interlaboratorio de bioensayos marinos para la evaluación de la calidad ambiental de sedimentos costeros en España. II. Ensayo de inhibición de la bioluminiscencia para la evaluación rápida de la toxicidad de sedimentos
- M.C. Casado Martínez 2
- T. Campisi 3
- A. Díaz 4
- R. Lo Re 5
- R. Obispo 6
- J.F. Postma 7
- I. Riba 1
- A.C. Sneekes
- T.A. DelValls 2
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1
Instituto de Ciencias Marinas de Andalucía
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2
Universidad de Cádiz
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- 3 Centro Ricerche e Servizi Ambientali Fenice
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4
GAIKER Centro Tecnológico
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- 5 Thetis Spa
- 6 CEDEX Centro de Estudios de Puertos y Costas
- 7 Aquasense Laboratories
ISSN: 0185-3880, 2395-9053
Year of publication: 2006
Volume: 32
Issue: 1
Pages: 129-138
Type: Article
More publications in: Ciencias marinas
Sustainable development goals
Abstract
The Microtox® bioassay was tested in an interlaboratory study to evaluate the variability when using solid-phase samples. The exercise consisted of two consecutive phases each one carried out with six sediment samples from Spanish ports. Phase I included six laboratories that reported results for the solid-phase test (SPT) protocol, while Phase II included seven laboratories for the SPT protocol and three laboratories for the basic solid-phase test (BSPT) protocol. Even though some interfering factors were identified that could affect the results and sample classification, the coefficients of variation (CV) can be considered in the range for biological tests. When these factors are considered for further assessments, interlaboratory variability is significantly reduced. The BSPT showed lower CV values than the SPT despite the small number of participating laboratories. This better performance may be explained either by the simplicity of the methodology.