Isolation and characterization of novel polymorphic microsatellite markers for the white stork, "Ciconia ciconia": applications in individual–based and population genetics

  1. S. Feldman 1
  2. A. Centeno-Cuadros 2
  3. R. Nathan 1
  1. 1 Hebrew University, Israel
  2. 2 Universidad Pablo de Olavide
    info

    Universidad Pablo de Olavide

    Sevilla, España

    ROR https://ror.org/02z749649

Journal:
Animal Biodiversity and Conservation

ISSN: 1578-665X

Year of publication: 2016

Volume: 39

Issue: 1

Pages: 11-16

Type: Article

DOI: 10.32800/ABC.2016.39.0011 DIALNET GOOGLE SCHOLAR lock_openOpen access editor

More publications in: Animal Biodiversity and Conservation

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Abstract

The white stork, Ciconia ciconia, is a model species for studies of bird migration and behavior, but previously published genetic markers are not informative enough to perform individual–based genetic studies. Following discovery using next generation sequencing, 11 polymorphic markers were selected and tested in samples from two study sites. The number of alleles per locus ranged from 2–10 with an average of 5.3. The mean observed and expected heterozygosities were 0.519 and 0.565 respectively. PID was adequately sensitive for population– and individual–based genetics studies. There was no significant evidence of allelic drop–out, null alleles, or other errors; one sample site deviated from Hardy–Weinberg equilibrium for two loci, but no loci deviated in both samples, suggesting utility of these markers. These markers can be used to answer a range of ecological questions including those related to genetic diversity, degree of natal philopatry, and genetic mating strategies.