Identificación de células organizadoras de tejido linfoide en amígdalas humanas

  1. Prados Martín, Alejandro
unter der Leitung von:
  1. Enrique García Olivares Doktorvater/Doktormutter
  2. Raquel Muñoz Fernández Co-Doktormutter

Universität der Verteidigung: Universidad de Granada

Fecha de defensa: 12 von April von 2013

Gericht:
  1. Antonio Tores Rueda Präsident/in
  2. María del Carmen Ruiz Ruiz Sekretär/in
  3. Irene Tirado Vocal
  4. Francisco Javier O'Valle Ravassa Vocal
  5. Natalia Aptsiauri Vocal

Art: Dissertation

Zusammenfassung

Secondary lymphoid organs (SLOs) originate during embryonic development through a close interaction between lymphoid tissue inducer cells (LTi cells) and lymphoid tissue organizers cells (LTo cells). This interaction results in the proliferation and maturation of both cell types, and the expansion of the SLO germinal primordium. Subsequently, B and T lymphocytes arrive to the primordium, the lymphoid microdomains are organized and LTo cells differentiate into Follicular Dendritic Cells (FDC), Marginal Reticular Cells (MRC) or Fibroblastic Reticular Cells (FRC), depending on the region of SLO. In this thesis, we obtain cell lines of tonsil stromal cells (TSC) and, for the first time in humans adult SLOs, we identified a cell population with a phenotype similar to murine LTo cells (Podoplanin+, CCL19+, CCL21+, CXCL13+, TRANCE+, ICAM-1+, VCAM-1+ and IL-7+), and, like LTo cells, they can also adhere and rescue from apoptosis to lymphoid cells. Moreover, these cell lines express mesenchymal stem cells (MSC) markers (CD10+, CD29+, CD44+, CD73+, CD271+, c-Kit+, Nestina+, NAGOG+, STRO-1+, and OCT-3/4+) and they can be differentiate into other mesenchymal lineage cell types.