Instrumental development for obtaining extended depth of field images: study of the influence of the chromatic coordinates used as basis of the calculation
- J. Navas 1
- M. Kulawik 1
- R. Alcántara 1
- C. Fernández-Lorenzo 1
- J. Martín-Calleja 1
- 1 Physical Chemistry Department. Science Faculty. University of Cádiz. 11510 Puerto Real, Cádiz (Spain).
Editorial: Formatex Research Center
ISBN: 978-84-614-6190-5
Año de publicación: 2010
Páginas: 1474-1482
Tipo: Capítulo de Libro
Resumen
One of the major limitations of the high resolution optical microscopy is the low depth of field. This limitation is caused by the radiation used as illumination, and it depends on the wavelength of the radiation. However, the use of combined techniques of digital image acquisition and powerful software can afford to get completely focused images. The images obtained with this technique can show a virtual depth of field increased by an order of magnitude. In this chapter, we will show the way for obtaining extended depth of field images using a complete system (instrumentation, algorithm and software) for acquiring micrograph images and with no alteration of the original chromaticity. The dependence of the final result is shown according to the chromatic components used as the variables on the algorithm designed, so we will show a methodology based on the study of second variance in relation to the number of images and pixels of the chromatic coordinates to decide which to use as the basis for the calculation.