A comparative evaluation of biaccumulation and induction of oxidative stress by waterborne Cu and As as dissolved salts and nanoparticles in larvae of European seabass.

  1. Canalejo Raya, A. 2
  2. Granado-Castro, M.D. 1
  3. Díaz de Alba, M. 1
  4. Espada-Bellido, E. 1
  5. Casanueva Marenco, M.J. 3
  6. Galindo Riaño, M.D. 1
  7. Torronteras, R. 2
  1. 1 Department of Analytical Chemistry. Faculty of Science. University of Cádiz. Cádiz.
  2. 2 Department of Integrated Sciences/ Research Center RENSMA, University of Huelva. Huelva.
  3. 3 Department of Analytical Chemistry. Faculty of Science. University of Cádiz. Cádiz
Mostrar afiliaciones +
Actas:
XIX Congress of Society of Celular Biology

Editorial: EMBO

Año de publicación: 2021

Páginas: 108

Tipo: Póster de Congreso

GOOGLE SCHOLAR

Resumen

Toxicity of metals in their dissolved (DS) forms have been broadly addressed, but information on that related to the nanoparticles (NP) forms is lacking. We assess oxidative stress and the antioxidant enzymatic response induced in Dicentrarchus labrax larvae by copper and arsenic under these different chemical forms. Larvae were grouped (50) in 1.5 L tanks and exposed to 0; 0.01; 0.1; 1 and 10ppm of CuSO4•5H2O; copper(II)-oxide-NP; As2O3, or As2S2-NPs for 24 or 96h. Metal concentrations were analysed by ICP-MS and oxidative stress biomarkers were spectrophotometrically evaluated. Larval Cu-concentrations (mg/Kg d.w.) ranged from 2.75 to 7.85; whereas As-concentrations ranged from 1.91 to 27.72. Cu-DS induced oxidative damage (increased hydroperoxides and MDA levels) from the 0.1ppm at both exposure periods, while for Cu-NP it was not observed until 1ppm; which was paralleled by concomitant increases in SOD activity. Increased CAT activity was observed since 0.01ppm and 0.1ppm for Cu-DS and Cu-NP, respectively. No oxidative damage was detected for any form of As after 24h; it was induced by As-DS since the lowest concentration after 96h. SOD decreased dose-dependently in As-DS after 24h, but increased after 96h; As-NP did not alter SOD activity. CAT activity was dose-dependently stimulated at 96h by As-DS and at 24h by As-NP. Therefore, a differential induction of oxidative damage and antioxidant response was observed for the two metals and their chemical forms. DS-forms tend to be more potent than the NP-forms.