LEAD NANOPARTICLES IMPACT AS EMERGING CONTAMINANTS IN SEA BREAM LARVAE (SPARUS AURATA) AFTER SHORT EXPOSURE

  1. M.J. Casanueva Marenco 2
  2. M.J. Rosado Rodríguez 2
  3. M.D. Granado Castro 2
  4. A. Canalejo Raya 3
  5. F. Córdoba García 3
  6. R. Torronteras Santiago 3
  7. M.D. Galindo Riaño 1
  8. M. Díaz de Alba 1
  1. 1 Department of Analytical Chemistry. Institute of Biomolecules (INBIO), Faculty of Science, CEI-MAR. University of Cadiz. Spain.
  2. 2 Department of Analytical Chemistry. Institute of Biomolecules (INBIO), Faculty of Science, CEI-MAR. University of Cadiz. Spain
  3. 3 Department of Environmental Biology and Public Health. Faculty of Experimental Sciences, CEI-MAR. University of Huelva. Spain.
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Actas:
International Conference on Environmental and Food Monitoring (ISEAC-40)

Editorial: Jose Benito Quintana, Cristina Nerin, Rosario Rodil

ISBN: 978-84-09-03799-5

Año de publicación: 2018

Páginas: 132

Tipo: Aportación congreso

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Resumen

Due to the inevitable development of nanotechnology, the presence of nanomaterials on theenvironment is increasingly important, so that they are already considered “potentially dangerousemerging contaminants”. One of the pollution sources are those caused by lead oxide nanoparticles fromindustrial activities, such as pigments, lead-acid bateries, catalysts, photovoltaic sensors…Thus, this work was proposed to address the impact of these emerging contaminants in aquaticecosystems with a marine macro- species, gilthead seabream (Sparus aurata) in its larval state atlaboratory scale, for their great commercial value and its extensive use in aquaculture. PbO Nanoparticles(NPs) were synthesized in the lab and the nanoparticle size and size-distribution was evaluated. A toxicitybioassay was carried out under laboratory conditions and the organisms were exposed to differentconcentrations of PbO NPs: 0 (control); 0.01; 0.1; 1 and 10 mg L-1 at different exposure times: 24 to 72hours, with two replicates. A set of biomarkers, including the malondialdehyde content, thehydroperoxides level and the antioxidant response of tissues by the enzymes catalase and superoxidedismutase activities were studied. The exposure to waterborne PbO NPs induced a significant oxidativestress, outstanding the stimulation of superoxide dismutase activity atier 24 h as well as the increase ofhydroperoxides and malondialdehyde levels. By contrast, the catalase activity atier 72 h was inhibited.Also, a NPs dose-dependency was associated with the antioxidant response and the metalbioaccumulation in larvae. These results showed the potential applicability of the parameters studied asreliable early biomarkers for monitoring lead nanoparticles pollution in seawater.